Unknowns – 1 identification of unknowns in this exercise, you will draw upon many of your new skills to identify unknown bacteria you will be given three bacterial cultures and asked to identify each to the level of genus. The unknown bacterium will be identified either by comparing the resulting mass spectra with a collective proteomics database containing mass spectra of known bacteria, or by searching and matching the sequence of a panel of proteins with proteins of known bacteria in the protein database. Characteristics of these cells is needed to identify the genus and species of the bacterium the metabolic capabilities of the organism are studied in more detail identifying an unknown is a fascinating process.
Identification of unknown bacteria it is virtually impossible to identify bacteria based on physical characteristics alone this is due to the fact that there are only a few basic shapes and physical features commonly seen in the. The reason for identification of unknown bacteria was to help students recognize different bacteria through different biochemical tests and characteristics this is important in the medical field because identification of unknown bacteria can help treat a patient by knowing the contributing source of a disease. Identifying the unknown bacterium was determined by separating and differentiating possible bacteria based on specific biochemical characteristics the differential tests used to identify the unknown cultures were gram staining, oxidase, indole test, urea test, and casein test.
The identification of unknown cultures is a practical application of a classification scheme, so that a new isolate can be recognized as a member of an existing species. Identifying unknown bacteria using biochemical and molecular methods beginning of instructor pages instructor pages - - 3 purpose the purpose of this lab is to introduce a variety of lab techniques to students working on the common problem of identifying an unknown bacterium. The correct identification for the gram positive bacterium was bacillus cereus the b cereus was found using the solution in the unknown 109 test tube originally, a sample from the unknown tube was supposed to be inoculated on a nutrient agar plate using the quadrant streak technique.
Lab #3b: identification of unknown bacteria part ii [ powerpoint show or pdf printout ] printing: the best way for students to print out the powerpoint show is to download the pdf version. The unknown gram positive bacteria is staphylococcus aureus some problems encountered on this activity is the handling and contamination due to a lot of plate and tubes in one incubator, some samples are missing. Before attempting to identify an unknown bacterial species, the organism must be isolated in a ___ culture pure which of the following is the quickest way to isolate bacteria from a mixture. Identifying unknown bacteria using biochemical and molecular methods credits: this lab was created by robert kranz, kathleen weston-hafer, and eric richards the lab was developed and written by kathleen weston-hafer. Begin the process of identifying unknown bacteria by observing their physical characteristics, such as cell wall, shape and linkages use standard laboratory procedures, like cell staining, culturing and dna sequencing to further narrow down your identification.
Gram negative or gram positive in order to identify my unknown species, i used good aseptic technique to separate bacteria on an isolation streak plate. The bacteria being utilized in this experimentation will be unknown gram negative bacterium number 22 that had been inoculated in a tryptic soy broth after receiving the unknown number 22, it was gram stained to be assured that the bacteria being utilized was indeed gram-negative. Many proteolytic bacteria cause this reaction in the if you are attempting to identify a gram-negative, first 24 hours facultative, rod-shaped bacterial organism, group these series of tests together in this manner to see how litmus reduction culture becomes white ac- your unknown fits this combination of tests. Identification of unknown bacteria it is virtually impossible to identify bacteria based on physical characteristics alone this is due to the fact that there are only a few basic shapes and physical features commonly seen. Before going over the tests to identify an unknown bacterial species, we should remember some bases of manipulating bacteria it is important to always keep in mind that your unknown species is a potential pathogen.
Commercially available solutions of antibodies against specific bacteria (antisera) are used to identify unknown organisms in a procedure called a slide agglutination test a sample of unknown bacteria in a drop of saline is mixed with antisera that has been raised against a known species of bacteria. Unknown lab report unknown number 109 tyler wolfangel april 29, 2014 bio 203-604 introduction the study of microbiology requires not only an academic understanding of the microscopic world but also a practical understanding of lab techniques and procedures used to identify, control, and manipulate microorganisms. Unknown lab report microbiology unknown a sonia kabra november 26, 2014 introduction there are numerous reasons for identifying unknown bacteria some of these organisms have distinct qualities that set them apart from one another, such as the exposure to certain environments. Identification matrix screen the bacteria supported and their results for all of the tests supported are kept in a matrix, or table you may display t he i dentification m atrix by selecting identification matrix from the view menu the i dentification m atrix shows your assigned unknown with the results you have recorded for it in the first column.
A faster approach to identifying bacteria the speedy turnaround time was a result of identification of the bacteria directly from the positive blood culture bottle using matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometry, an innovative technology that offers a significant improvement over bacterial. Perform a gram stain on your organism just like last time, when gram staining an unknown, the best method is to make three bacterial smears on the same slide. This project was created with explain everything ™ interactive whiteboard for ipad.